EFFICIENCY OF PSYCHIC SELF-REGULATION, EMOTIONAL STABILITY AND STRESS-VULNERABILITY OF ATHLETES-TENNIS PLAYERS BY THE METHOD OF CHOOSING COLORS IN CONNECTION WITH THE FUNCTIONAL STATE OF THE NEUROMUSCULAR APPARATUS

Выявлена взаимосвязь электронейромиографических и психологических характеристик у спортсменов-теннисистов: более высокой скорости проведения нервного импульса соответствовали более низкий уровень стресса, более высокий уровень эффективности психической саморегуляции и адаптивности, более высокий уровень эмоциональной устойчивости. Установлена статистически значимая положительная корреляция между коэффициентом вегетативного баланса по тесту М. Люшера и скоростью проведения нервного импульса по моторным волокнам срединного нерва верхней конечности, что может быть обусловлено функциональным состоянием сердечно-сосудистой системы. Полученные результаты могут быть использованы для коррекции тренировочного процесса молодых спортсменов-теннисистов. Ключевые слова: электронейромиография, скорость проведения нервного импульса, уровень стресса, психическая саморегуляция, адаптивность, эмоциональная устойчивость, спортсмены, теннис.

Introduction. Increased formation of active forms of oxygen, increase of the level of products of free radical lipid peroxidation oxidation and decrease in the activity of antioxidant systems is observed in the pathogenesis of many diseases, in particular during the development of malignant neoplasms [1].
It is known that free radicals are one of the carcinogenic factors. They are present at all stages of the development of the tumor and have high reactivity and can cause damage to the lipid bilayer of cell membranes and directly to DNA molecules [1,2].
Lipoperoxides are quite unstable, and are subjected to further oxidative degeneration. In this case, accumulate secondary oxidation products, the most important of which are malonic dialdehyde (MDA) [3].
Accumulation of lipid peroxidation (LPO) products in the body and the development of endotoxicosis leads to stimulation of the monooxygenase system, changes in lipid, hormonal, immune, micronutrient, neurotransmitter status, number of binding sites and affinity of receptors to ligands, and depletion of the antioxidant system [4]. Previously, we showed a significant difference in the action of photosensitive peptidomimetics on tumor cell models in vitro in vivo [5]. However, in contrast to the traditional photo of dynamic therapy with irradiation in a narrow range of wavelengths, a photo-sensitive peptidomimetic is activated by visible light, so it is likely that the launch of cell death occurs without the formation of active forms of oxygen. To check this hypothesis, it was important to evaluate lipid peroxidation. Therefore, the purpose of our work was to determine the effect of photosensitive peptidomimetik (FPM or PM) on the content of lipid peroxidation products in the mouse lymphoblast cell line L1210. The tasks of the work were: -Determine how the closed and open form of FPM affects the level of TBA-active products.
-Determine how the level of diene, triene conjugates and Schiff bases changes under the action of test substances.
-Determine the effect of FPM on the number of cells in a proliferative pool.

Materials and methods.
The study used cell lines L1210 (suspension, mouse lymphoblastoma, lymphocytic lymphoma) cultured under standard conditions in an incubator at 37°C, 5 % CO2, DMEM medium. The number of cells counted in the hemocytometer. The protein level was determined using a set of reagents of the "File-Diagnostics" Ltd. The content of schiff bases, diene conjugates, trienic conjugates of unsaturated fatty acids of neutral lipids and phospholipids was determined according to standard techniques on a spectrophotometer at λ = 400, 232, 278, 220 nm, respectively [6]. The neutral lipids and phospholipids was separate by heptane and isopropyl fraction. The content of TBA-active products (MDA) was determined on a spectrophotometer at λ = 532 nm [7]. The antiproliferative effect was determined by flow cytofluorometry. Experimental data was processed according to generally accepted statistical methods.
Results and discussionю. We obtained the following results for the action of the open and closed forms of peptidomimetics: there are statistically insignificant fluctuations of the average value of the content of diene conjugates has not changed with respect to control in both phases and states of FPM (Fig. 1); the same thing was observed in the determination of trienic conjugates (Fig. 2); the content of TBA-active products in cells relative to control (Fig. 3); In the investigation of the content of Schiff bases, a statistical difference was observed only in the phospholipid phase with an open form of PM, in the neutral (open and closed forms of PM) and the closed form in the phospholipid phases have statistically insignificant changes (Fig. 4).

Fig. 1. The content of diene conjugates in the fraction of neutral lipids (A) and phospholipids (B) of mouse lymphoblast cells of L1210 by the action of the test substances (Mm, n = 5)
*р < 0,05 in comparison with control. With all of the above, in the L1210 cells, a statistically significant increase in death through apoptosis was observed, both relative to the control group and the groups of cells that were incubated with open and closed forms of PM with each other (table 1).  Conclusions. As a result of our studies, the content of lipid peroxidation oxidation products in the mouse lymphoblast cells of L1210 was determined by the action of a photosensitive peptidomimetic. It was found that in cells that were cultured with the addition of an open and closed form of peptidomimetic GS-DProSw, no preexisting changes in the level of malondialdehyde were observed. It was determined that the photosensitive peptidomimetics does not show an increase in the level of Schiff bases, diene and trienic conjugates. It has been shown that the highest level of cell apoptosis was observed due to the exposure of the open form of the peptidomimetic GS-DProSw. The most prominent cytotoxic and cytostatic action is the open form of the photosensitive peptidomimetic GS-DProSw. Consequently, this allows us to conclude that the mechanism of action of substances does not involve active forms of oxygen to neutralize cells.